ABSTRACT
【Objective】 To design and manufacture a non-destructive intestinal decompression device and explore its effect on closed intestinal decompression in patients with intestinal obstruction. 【Methods】 A total of 78 patients with intestinal obstruction who underwent intestinal decompression in our hospital from January 2020 to September 2021 were selected as the research subjects: 40 in the control group and 38 in the experimental group. The traditional intestinal decompression method was used in the control group while the non-invasive intestinal decompression device method was used in the experimental group. We compared the number of cases of intestinal content pollution, drainage volume of intestinal content decompression, operation time, hospital stay, incidence of complications, and other indexes between the two groups. 【Results】 The two groups did no significantly differ in the general data (P>0.05). There were 10 cases (26.32%) of intestinal content pollution in the experimental group and 40 cases (100%) in the control group. The intestinal decompression drainage volume in the experimental group was 750.00 (728.75, 827.50) mL and 535.00 (520.00557.50) mL in the control group, with significant difference between the two groups (P<0.05). The operation time, operation time and hospital stay were significantly shorter in the experimental group than in the control group (P<0.05). There was no incision infection in the experimental group but 6 cases in the control group, with statistically significant difference (P<0.05). There was no significant difference between the two groups in other abdominal infection, anastomotic fistula, or enterostomy-related complications (P>0.05). 【Conclusion】 The non-invasive intestinal decompression device can perform closed intestinal decompression in the operation of intestinal obstruction, reduce the pollution of the operation field, shorten operation time and hospital stay, and reduce the incidence of incision infection.
ABSTRACT
Laparoscopic proximal gastrectomy (LPG) can be selected for the treatment of early upper gastric carcinoma, but gastroesophageal reflux after operation would seriously affect the quality of life of patients. Esophagogastric anastomosis with double flap technique is a digestive tract reconstruction method using the anastomosis between the esophagus and the anterior wall of the stomach. Compared with other digestive tract reconstruction methods, esophagogastric anastomosis with double flap technique can maintain the postoperative body mass of patients in good condition, improve the nutritional status and the long-term quality of life of patients. Esophagogastric anasto-mosis with double flap technique has good anti reflux effects and retain the possibility of endoscopic examination and treatment. By reviewing literatures at home and abroad, and combined with clinical experiences, the authors discuss current status and digestive tract reconstruction methods of LPG, and deeply investigate the application prospect of esophagogastric anastomosis with double flap technique.
ABSTRACT
Laparoscopic radical gastrectomy is currently recognized as the standard surgical procedure for radical gastric cancer. The dissection of the sixth group of lymph node, that is the inferior pylorus region lymph node, should be performed step-by-step according to standard procedures, and surgeon on left position of patient is more conducive to the lymph node dissection of this area. The 4K laparoscopic system can increase the resolution of the surgical field of view, which provide the surgeon with a clear surgical field of vision, thereby improving the accuracy of operation, achieving the best requirements of lymph node dissection and avoiding side injury and complica-tions. The authors investigate infrapyloric lymph node dissection with the surgeon on left position in 4K laparoscopic radical gastrectomy.
ABSTRACT
Laparoscopic radical gastrectomy is currently recognized as the standard surgical procedure for radical gastric cancer. The dissection of the sixth group of lymph node, that is the inferior pylorus region lymph node, should be performed step-by-step according to standard procedures, and surgeon on left position of patient is more conducive to the lymph node dissection of this area. The 4K laparoscopic system can increase the resolution of the surgical field of view, which provide the surgeon with a clear surgical field of vision, thereby improving the accuracy of operation, achieving the best requirements of lymph node dissection and avoiding side injury and complica-tions. The authors investigate infrapyloric lymph node dissection with the surgeon on left position in 4K laparoscopic radical gastrectomy.
ABSTRACT
Objective To observe the Pin1 expression pattern in skin and to establish an inducible skin specific Pin1 overexpression mouse model. Methods The mouse Pin1 gene was cloned into modified vector pTRE2 with C?terminal Myc tag. The linearized pTRE2?Pin1 DNA was micro?injected into one?cell embryos followed by implantation into foster mice to produce TRE?Pin1 transgenic mice. Results TRE?Pin1 transgenic founder mice were successfully created. These mice were crossed with transgenic tool mice K14?rtTA to create epithelial specific double transgenic progenies. Pin1 gene was induced by incorporating doxycycline into drinking water of the mice. Pin1 protein overexpression in the skin was con?firmed by Western blot and immunohistochemistry. The endogenous Pin1 protein was predominantly expressed in epidermal cells in the skin. Conclusions The inducible skin specific Pin1 overexpression mouse model is successfully established which may serve as a useful model for further study of Pin1 functions in the skin.
ABSTRACT
Objective To compare and evaluate the efficacy of diagnosis and excision for appropriately selected breast multi-focal lesions and solitary lesion by ultrasound-guided vacuum-assisted biopsy (UGVAB). Methods Among 392 appropriately selected patients, 187 patients with multi-focal lesions and 205 patients with solitary lesion were treated by the 8-gauge UGVAB from May 2007 to June 2009. All lesions were removed as completely as possible. The patients with benign pathology underwent physical and ultrasound examinations at one week and 6 months after procedure. Results During the procedure, only three patients had vasovagal syncope and twenty others complained of other intraoperative discomfort. An accurate pathological diagnosis was obtained in all lesions. There was no apparent false-negative result among the 696 lesions with benign pathology at a follow-up of 6 months after procedure. The rates of malignant or premalignant pathology, postoperative complications and residual lesions in patients with multi-focal lesions were higher than those in patients with solitary lesion. If each lesion was considered as a subject of study, there was no significant difference between the two groups. Conclusion UGVAB is an effective method for diagnosis and excision of appropriately selected breast multi-focal lesions and can be used routinely.
ABSTRACT
Objective To study the synergistic effects of Mitomycin C in photodynamic therapy for colonic carcinoma. Methods The colon carcinoma cell line SW800 was assigned to be treated with 002 ?g/ml Mitomycin C, or 2 ?g/ml PSD-007 (photosensitizer), or Mitomycin C and PSD-007 (PSD+MC group).The cells without any special treatment served as control. Fluorescence spectrophotometer and MTT assay were used to detect the content of intracellular photosensitizer and survival rate of SW700 cells under photodynamic therapy. The obtained data was analyzed with SPLM statistical software. Results After PSD was added for 4, 8, 16 and 24 h, intracellular PSD level of PSD+MC group increased significantly as compared with that of PSD group. The intracellular PSD contents of PSD group, PSD+MC group had a strong and negative correlation with cell survival rate respectively after photodynamic therapy, but the cell survival rate curve shifted to the left obviously. The best killing effects on SW700 cells of photodynamic therapy could be obtained when the SW700 cells had been exposed to photosensitizer for 8-16 h. Conclusion There is insignificant influence of lowconcentration of Mitomycin C alone on cell survival rate. The better synergistic effect of Mitomycin C on killing the colon carcimoma cells can be achieved by enhancing the intracellular content of photosensitizer for 6-8 h before photodynamic therapy.
ABSTRACT
Objective To investigate the risk factors of complication rate after colorectal cancer operation.Methods This study recruited a total of 254 non-emergency colorectal cancer patients admitted to our hospital between December 2005 and December 2006,and then evaluated the effects of life style,preoperative factors and intraoperative factors on postoperative complications.Results Single factor analysis showed that the postoperative complication rate was not significantly affected by gender,age,obesity,palliative/curative resection,anesthesia style as well as preoperative drinking or smoking history.Preoperative complications(P=0.001),tumor stage and operation time(P=0.025) affected the postoperative complicatin rate.Multi-factor logistic regression analysis showed that preoperative complications were the only risk factor of postoperative complications [P=0.001,OR=5.871,(95% CI 2.958-11.651)].Conclusion Old age as such does not represent a contraindication for surgical treatment.Preoperative complications,operation time and tumor stage significantly affect the postoperative complication rate.Preoperative complications are the strongest risk factor of all.Therefore,reasonable perioperative managements and shortening operation time are the key to reducing postoperative complications.